You can feed multiple mice at one sitting with no problem. I have a male ball that's about 4 feet long. He gets 2-3 large mice, occassionally 4 if one of the other mouse eatters doesn't finish theirs, per feeding. Once one mouse is swallowed I place the next one in the enclosure. It doesn't stress them to eat their prey at one sitting. Also, after they have eatten, it is best to allow them to digest and not handle them for a day or two. With feeding them at one time, you are not stretching the feedings out and they can digest in peace before being handled.